Analytical HPLC

Following are links to publications on this website featuring analytical applicatIons of Vydac HPLC columns and related issues.
 

Application Notes:

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#9602: Protein Digests: a Comparative Analysis
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#9701: The Influence of C18 Ligand Type on the Peptide Selectivity of Silica-Based Reversed-Phase Columns
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#9703: Developing a Robust Reversed-Phase Method for the Analysis of Polypeptides
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#9804: What is 0.1% TFA?
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#9805: Are Ghost Peaks Haunting Your Chromatograms?
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#9806: Separating Complex Carbohydrates
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#9810: Nucleotide Analysis with Vydac 302IC4.6 Ion-Chromatography Columns
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#9811: Assessing Polypeptide Integrity: Reversed-Phase HPLC vs. PAGE
& Reversed-Phase Column Cleaning: SDS is OK!
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#9812: Taxol and Related Products
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#9902: Fast Protein and Peptide Analyses with Vydac 3µm 300Å Reversed-Phase Columns
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#9904: Vitamin Analysis with Vydac Reversed-Phase Columns



Newsletter Articles:

Spring 2001 Article #1: Separating Small Molecules on 300 Å Reversed Phase
Article #4: Vydac Adsorbent Aids in LC/MS/MS Analyses
Article #5: Modifier Effects in Peptide Separations
Winter 2001 Article #1: Vydac's Contribution to Biopharmaceutical Development
Article #2: Vydac Offers New Capillary Columns for LC/MS
Article #3: What's in an Herb? Nutraceutical Analysis by HPLC
Article #5: Environmental Analyses:Diquat, Paraquat Herbicides & Acrylamide, Acrylonitrile, Acrolein (Propenal)
Summer 2000 Article #1: Peptide Separations: All 300 Å Reversed-Phase Columns are Not the Same
Article #2: Contributed Article: Direct Gradient Elution of Peptides for Capillary LC/MS/MS
Article #4: EPA Methods 531 (pesticides) and 8330 (explosives)
Spring 2000 Article #5: SelectaPore 300P is C18 Reversed-Phase Column of Choice for USP Analyses
Fall 1999 Article #1: New! Three SelectaPore C18 Columns for Pharmaceutical and Small-Molecule Analyses
Article #2: SelectaPore Column Evaluated for Cat's Claw Analysis
Article #3: Peptide Separations on a C18 LC/MS Column: Optimizing the Effects of Mobile-Phase Modifiers
Summer 1999 Article #1: Vydac Introduces New SP (Small-Pore) Silica for Pharmaceutical and Vitamin Analysis
Article #2: Separation of Barbiturates on Small-Pore and Wide-Pore Reversed-Phase Columns
Article #4: New LC/MS columns perform well with no TFA! High-performance separations of tryptic-digest peptides in 5mM HOAc
Spring 1999 Article #1: Two New Reversed-Phase Columns Permit Peptide Separations at Low TFA Concentrations: Beneficial for Applications Including Electrospray MS Analysis
Article #2: Separation of Fluorinated Taxol Analogs
Fall 1998 Article #1: Vydac 300Å Reversed-Phase Columns: Ideal for Packed-Column SFC
Article #3: Anion-Exchange of Phospholipids
Article #5: Herbal Extracts
Summer 1998 Article #2: New: Microbore Guards Available
Article #5: New Test Improves Carbon Loading Precision
Spring 1998 Article #1: Protein and Peptide LC/MS and Microanalysis Applications: Vydac Microbore Columns
Article #4: Analyzing Fat-Soluble and Water-Soluble Vitamins
Winter 1998 Article #1: Vydac 3µm 300Å Reversed-Phase Columns Speed Protein and Peptide Analyses
Article #2: Reversed-Phase Column Cleaning: SDS is OK!
Article #5: Vydac Ion-Chromatography Column Proves Ideal Tool for Nucleotide Analysis
Fall 1997 Article #1: Separating Complex Carbohydrates
Article #5: Assessing Polypeptide Integrity: Reversed-Phase HPLC vs. PAGE
Article #6: More Protein Characterization - Coupling laser light scattering detection with reversed-phase chromatography
Summer 1997 Article #2: Separation of Taxol and Related Products
Article #3: Effects of Column Length in Peptide Ion Exchange
Article #4: Vydac's Unique Silica
Article #5: Troubleshooting and Eliminating Ghost Peaks
Article #6: Separating t-RNA Mixtures
Spring 1997 Article #1: New Tools for Peptide Separations
Article #2: Selectivity Effects of TFA Concentration
Article #4: Virtually Indestructible Reversed-Phase

Posters:

Low-TFA Reversed-Phase Capillary Columns for LC/MS
Ning Mu and Kervin Harrison, Vydac/The Separations Group, and Susan T. Weintraub, Dept. of Biochemistry, University of Texas Health Science Cener at San Antonio
presented at HPLC 2001 / Maastricht, The Netherlands / June 17-22, 2001 and AAPS Annual Meeting / Denver, CO / October 21-25, 2001

The Significance of pH, Counter Ions, Bonding Chemistry and Pore Size in Silica-based Reversed Phase Method Development for Pharmaceuticals
Michael K. Li, Vydac/The Separations Group, Inc.
presented at Pharmaceutical Conference of the Americas / Orlando, FL, USA / March 25-29, 2001

LC/MS Reversed-Phase Columns with Excellent Polypeptide Resolution in Low TFA Concentrations
M.K. Li and P.J. Kostel, Vydac/The Separations Group, Inc.
presented at ABRF / Durham, NC, USA / March 19-22, 1999

Practical Aspects for the Development of a Reversed-Phase Analytical Method for Bio-Pharmaceuticals
Paul Kostel and Michael Li, Vydac/The Separations Group, Inc.
presented at the 3rd International Symposium on the Analysis of Well-Characterized Biotechnology Pharmaceuticals, Washington, DC, USA / January 5-8, 1999



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