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Particulate and chemical contaminants entering or arising in the HPLC system can adversely affect column performance. The effects of column contamination may appear as high back-pressure, reduced column life, peak broadening, or changes in retention and selectivity. Contaminants in an HPLC system are typically traceable to either of two sources:
Problem: Fine particulate matter from abrasion of pumping system and mixer parts can reach the column causing high column back-pressure. Routine filtration of solvents during preparation and at the point of withdrawal from reservoirs is a good practice. However, it does not protect against particulates generated in the solvent delivery system.
Solution: Place an in-line eluent filter between the solvent delivery system and the sample injector. Vydac
offers an in-line eluent filter (Catalog No. EF-4) consisting of a high-surface-area 2µ metal element in a holder that can
be connected to any HPLC system. The eluent filter removes particulate contaminants from the solvent.
Problem: Insoluble material in the sample or sample components which are strongly retained on the column matrix can reduce column lifetime by causing high column back-pressure or changing column selectivity.
Solution: Vydac offers high-performance guard columns designed to protect your column from insoluble
or strongly retained sample components. To remove sample components which may damage the column, install
a Vydac High-Performance Guard Column between the sample injector and the column.
Vydac offers a High-Performance Guard Column system consisting of a reusable holder and replaceable cartridge containing the same separation material used in the column. Cartridges are replaced as needed and the holder can be used indefinitely. The Vydac High-Performance Guard Holder with cartridge is placed between the sample injector and the column. High performance guard columns retain and, in some cases, enhance column performance. High-Performance Guard Columns are available for Vydac columns from 1.0 mm to 22 mm diameter.
| Guard column kits | Replacement cartridges | ||
|---|---|---|---|
| Include a holder and one cartridge | Two cartridges per pack | ||
| Catalog numbers
xxxGK51 xxxGK52 xxxGK54 |
|
Catalog numbers xxxGD51 xxxGD52 xxxGD54 |
|
NOTE: When ordering, replace "xxx" with specific 3-digit packing type designation. (See complete listing below.)
| Guard column kits | Replacement cartridges | ||
|---|---|---|---|
| Include a holder and one cartridge | One cartridge per pack | ||
| Catalog numbers xxxFSK1210 |
 |
Catalog numbers xxxGCC1210 |
 |
NOTE: When ordering, replace "xxx" with specific 3-digit packing type designation. (See complete listing below.)
Each guard kit consists of a cartridge holder and one cartridge.
Replacement guard cartridges are shipped two per box for microbore (1.0 mm ID), narrow-bore (2.1 mm ID) and analytical (4.6 mm ID) cartridges, one per box for semi-preparative (10 mm ID) cartridges.
| Phase | Particle Size | Cartridge Inside Diameter | Guard Kit Cat. No. | Replacement Cartridges Cat. No. | For use with the following columns |
|---|---|---|---|---|---|
 | |||||
| 201TP | 5 micron | 2.1 mm | 201GK52T | 201GD52T | 201TP52, 201TP5215 |
| 5 micron | 4.6 mm | 201GK54T | 201GD54T | 201TP54, 201TP5415, 201TP510 | |
| 10 micron | 4.6 mm | 201GK104T | 201GD104T | 201TP104, 201TP1010 | |
| |||||
| 202TP | 5 micron | 4.6 mm | 202GK54T | 202GD54T | 202TP54, 202TP5415 |
| 10 micron | 4.6 mm | 202GK104T | 202GD104T | 202TP10405 | |
| |||||
| 208TP | 5 micron | 1.0 mm | 208GK51 | 208GD51 | 208TP51, 208TP5115, 208TP5105 |
| 5 micron | 2.1 mm | 208GK52 | 208GD52 | 208TP52, 208TP5215, 208TP5205 | |
| 5 micron | 4.6 mm | 208GK54 | 208GD54 | 208TP54, 208TP5415, 208TP5405, 208TP510 | |
| 10 micron | 4.6 mm | 208GK104 | 208GD104 | 208TP104, 208TP10415, 208TP1010 | |
| 12 micron | 10 mm | 208FSK1210 | 208GCC1210 | 208TP1010, 208TP1022, 208TP101522, 208TP152022 | |
| |||||
| 214MS | 5 micron | 1.0 mm | 214GK51MS | 214GD51MS | 214MS51, 214MS5115, 214MS5105 |
| 5 micron | 2.1 mm | 214GK52MS | 214GD52MS | 214MS52, 214MS5215, 214MS5205 | |
| 5 micron | 4.6 mm | 214GK54MS | 214GD54MS | 214MS54, 214MS5415, 214MS5405, 214MS510 | |
| |||||
| 214TP | 5 micron | 1.0 mm | 214GK51 | 214GD51 | 214TP51, 214TP5115, 214TP5105 |
| 5 micron | 2.1 mm | 214GK52 | 214GD52 | 214TP52, 214TP5215, 214TP5205 | |
| 5 micron | 4.6 mm | 214GK54 | 214GD54 | 214TP54, 214TP5415, 214TP5405, 214TP510 | |
| 10 micron | 4.6 mm | 214GK104 | 214GD104 | 214TP104, 214TP10415, 214TP1010 | |
| 12 micron | 10 mm | 214FSK1210 | 214GCC1210 | 214TP1010, 214TP1022, 214TP101522, 214TP152022 | |
| |||||
| 218MR | 5 micron | 4.6 mm | 218GK54M | 218GD54M | 218MR54, 218MR5415, 218MR5405, 218MR510 |
| 10 micron | 4.6 mm | 218GK104M | 218GD104M | 218MR104, 218MR10415, 218MR1010 | |
| |||||
| 218MS | 5 micron | 1.0 mm | 218GK51MS | 218GD51MS | 218MS51, 218MS5115, 218MS5105 |
| 5 micron | 2.1 mm | 218GK52MS | 218GD52MS | 218MS52, 218MS5215, 218MS5205 | |
| 5 micron | 4.6 mm | 218GK54MS | 218GD54MS | 218MS54, 218MS5415, 218MS5405, 218MS510 | |
| |||||
| 218TP | 5 micron | 1.0 mm | 218GK51 | 218GD51 | 218TP51, 218TP5115, 218TP5105 |
| 5 micron | 2.1 mm | 218GK52 | 218GD52 | 218TP52, 218TP5215, 218TP5205 | |
| 5 micron | 4.6 mm | 218GK54 | 218GD54 | 218TP54, 218TP5415, 218TP5405, 218TP510 | |
| 10 micron | 4.6 mm | 218GK104 | 218GD104 | 218TP104, 218TP10415, 218TP1010 | |
| 12 micron | 10 mm | 218FSK1210 | 218GCC1210 | 218TP1010, 218TP1022, 218TP101522, 218TP152022 | |
| |||||
| 219TP | 5 micron | 1.0 mm | 219GK51 | 219GD51 | 219TP51, 219TP5115, 219TP5105 |
| 5 micron | 2.1 mm | 219GK52 | 219GD52 | 219TP52, 219TP5215, 219TP5205 | |
| 5 micron | 4.6 mm | 219GK54 | 219GD54 | 219TP54, 219TP5415, 219TP5405, 219TP510 | |
| 10 micron | 4.6 mm | 219GK104 | 219GD104 | 219TP104, 219TP10415, 219TP1010 | |
| 12 micron | 10 mm | 219FSK1210 | 219GCC1210 | 219TP1010, 219TP1022, 219TP101522, 219TP152022 | |
| |||||
| 238TP | 5 micron | 4.6 mm | 238GK54 | 238GD54 | 238TP54, 238TP5415, 238TP5405, 238TP510 |
| 10 micron | 4.6 mm | 238GK104 | 238GD104 | 238TP104, 238TP10415, 238TP1010 | |
| |||||
| 302IC | 10 micron | 4.6 mm | 302GK4 | 302GD4 | 302IC4.6 |
| |||||
| High-Performance Guard Cartridge Holders only | Description | Cat. No. | For use with |
|---|---|---|---|
| Analytical Holder | GCH4 | 2.1 and 4.6 mm ID cartridges | |
| Semi-Preparative Holder | GCH10 | 10 mm ID cartridges | |
| |||
| In-line Mobile-Phase Filter | Description | Cat. No. | Contains |
| Eluent Filter | EF-4 | Holder and two filter elements | |
| Replacement Filter Element | EF-4RF | Two replacement filter elements per package | |