Vydac 238TP C18 reversed-phase material consists of octadecyl aliphatic groups bonded to the surface of 300 angstrom pore diameter silica. The large pores of the 300 angstrom TP silica allow polypeptide molecules complete access to the interior surfaces. For Vydac's 238TP adsorbent, the hydrophobic (reversed) phase is attached using monofunctional octadecylchlorosilanes, resulting in a uniform monomeric layer of C18 groups. After end-capping, the resulting reversed-phase material has excellent stability and reproducibility and provides subtle differences in selectivity compared to the 218TP polymeric C18 adsorbent. These selectivity differences can be exploited to separate compounds that are difficult to resolve on one C18 column alone and to aid in identification of more components in complex mixtures, for example partial digests of proteins and polysaccharides.
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