2. How do I protect my HPLC columns?

              3. Is TFA the only Reverse Phase Buffer for  
                  Proteins and Peptides?                 

              4. How do I clean my C4 HGH column?

              5. What effect does flow rate have on my RP-HPLC
                   column?

              6. What effect does column length have on my
                  RP-HPLC column?

              7. How much polypeptide can be purified in one
                  RP-HPLC run?

              8. What effect do surfactants have on RP-HPLC
                  separations?

              9. Does RP-HPLC of polypeptides affect biological
                  activity?

            10. What are the recommended flow rates for my
                  VYDAC columns?